EpigenDx Sample Submission Guidelines and Reference File Download Link
https://eu2.contabostorage.com/00f3241116844f24b628f46d81abb929:st1/folder11/11760/13282_sample_submission_form.doc
2026-06-02 01:00:16 - Admin
<style> body { font-family: Arial, sans-serif; line-height: 1.6; color: #333; max-width: 800px; margin: 40px auto; padding: 0 20px; background-color: #ffffff; } h1 { color: #2c3e50; border-bottom: 2px solid #3498db; padding-bottom: 10px; } h2 { color: #2980b9; margin-top: 30px; } ul { margin-left: 20px; } li { margin-bottom: 10px; } .highlight { background-color: #f9f9f9; padding: 15px; border-left: 5px solid #3498db; }</style><h1>EpigenDx Sample Submission Guidelines</h1><p>To ensure the highest quality of service and accuracy in epigenetic analysis, EpigenDx requires all clients to follow specific protocols regarding sample preparation, storage, and shipping. Adhering to these guidelines minimizes the risk of sample degradation and ensures that our laboratory processes your specimens efficiently.</p><h2>Sample Types and Preparation</h2><p>EpigenDx accepts a wide variety of biological materials, including genomic DNA (gDNA), fresh frozen tissue, FFPE (formalin-fixed paraffin-embedded) tissue, and blood samples. Proper preparation is critical for downstream applications like Pyrosequencing, methylation arrays, and next-generation sequencing.</p><ul> <li><strong>Genomic DNA:</strong> Samples should be dissolved in nuclease-free water or 10 mM Tris-HCl (pH 8.0-8.5). Please ensure that DNA is free of contaminants such as proteins, salts, or ethanol.</li> <li><strong>Tissue Samples:</strong> Fresh tissue should be snap-frozen in liquid nitrogen immediately after collection and stored at -80C.</li> <li><strong>FFPE Samples:</strong> When submitting FFPE blocks or sections, please provide high-quality slides with adequate surface area for DNA extraction.</li></ul><h2>Quantification and Quality Control</h2><p>Before submitting samples, please provide an accurate measurement of concentration and purity. We recommend using fluorometric quantification (e.g., Qubit) rather than spectrophotometry (e.g., NanoDrop) for more precise concentration readings, especially for low-yield samples.</p><div class="highlight"> <strong>Key Metrics for Submission:</strong> <ul> <li><strong>A260/280 Ratio:</strong> Should be between 1.8 and 2.0.</li> <li><strong>Concentration:</strong> Minimum volume and concentration requirements vary by service; please consult your specific project quote for exact details.</li> <li><strong>Integrity:</strong> For applications requiring high-molecular-weight DNA, gel electrophoresis images should be included.</li> </ul></div><h2>Labeling and Packaging</h2><p>Clear identification is essential to prevent processing errors. Each tube or plate must be clearly labeled with a unique Sample ID that matches your digital submission manifest.</p><ul> <li><strong>Labeling:</strong> Use permanent, cryo-resistant markers. Clearly write the sample name on the cap and the side of the tube.</li> <li><strong>Tube Security:</strong> Ensure caps are sealed tightly to prevent evaporation or contamination during transit. For plates, use high-quality adhesive seals.</li> <li><strong>Organization:</strong> Arrange samples in a logical order (e.g., numerical or alphabetical) within racks or storage boxes.</li></ul><h2>Shipping Instructions</h2><p>The stability of your samples is our priority. Please ensure that shipping conditions are appropriate for the specific sample type:</p><ul> <li><strong>Frozen Samples:</strong> Must be shipped on dry ice. Ensure there is a sufficient amount of dry ice to last at least 48-72 hours to account for potential carrier delays.</li> <li><strong>Ambient/Cold Samples:</strong> If shipping DNA in liquid form, use cold packs and insulate the container.</li> <li><strong>Documentation:</strong> Always include a printed copy of the Sample Submission Form inside the shipping package. This document should contain your contact information, the project ID, and a detailed list of all samples included in the shipment.</li></ul><h2>Communication and Project Coordination</h2><p>Prior to shipping, we ask that you notify our team of the expected arrival date and provide the tracking number. This allows our laboratory staff to be prepared for receipt and ensures that the samples are placed into storage immediately upon arrival.</p><p>If you have any questions regarding these guidelines or if you are dealing with unique sample types, please contact your project manager or the EpigenDx client services team before beginning the submission process. Adhering to these steps facilitates a smooth workflow and provides the best possible foundation for high-quality epigenetic data.</p>