FACS Sample Submission Form: A Comprehensive Guide
Flow Cytometry (FACS) is a powerful technology used to analyze and sort cells based on their physical and chemical characteristics. To ensure high-quality data and maintain the integrity of the cytometry instrumentation, core facilities require researchers to complete a formal Sample Submission Form for every session. This process is essential for logistical planning, biosafety compliance, and technical optimization.
Why the Submission Form is Essential
The submission form serves as the primary communication bridge between the researcher and the facility manager. By providing detailed information, you help the technical team prepare the instrument, configure the lasers, and ensure that the appropriate safety protocols are in place before you arrive.
Key Information Required
When preparing your FACS submission, you should be prepared to provide the following details:
- Principal Investigator (PI) Information: The account holder responsible for the study and billing.
- Sample Source: Identification of the cell type, tissue origin, and species.
- Biosafety Level (BSL): Clear documentation of the biosafety level (BSL-1 or BSL-2) to ensure compliance with institutional regulations.
- Fluorochrome Panel: A list of all fluorochromes used in your experiment to confirm they align with the current laser configuration of the sorter.
- Cell Concentration and Volume: Estimates of cell density are critical to prevent clogging and to calculate the time required for sorting.
- Buffer Composition: Information on the media or buffers used, as these can affect the fluidics of the machine.
Preparing Your Samples
Beyond filling out the form, proper sample preparation is the most significant factor in a successful experiment. Ensure that your cells are filtered through a 3050 micron mesh immediately prior to submission to remove clumps. Aggregated cells are the leading cause of instrument clogs and inconsistent data.
Scheduling and Turnaround
Submission forms should typically be submitted at least 48 hours in advance. This allows the core facility staff to perform quality control, verify panel compatibility, and troubleshoot any potential issues before your scheduled time. Always check your facilitys specific online portal or manual for lead-time requirements.
Data Management and Collection
The submission form often includes a section regarding how you wish to collect your data. Whether you are sorting into tubes, plates, or slides, specify your collection requirements clearly. This ensures that the appropriate adapters and collection vessels are calibrated and ready upon your arrival.
Best Practices for Success
- Double-check your fluorochrome excitation and emission spectra against the facility's available lasers.
- Always include appropriate control samples (unstained cells, single-stain controls) as indicated on your form.
- Maintain accurate records of your concentrations to ensure the flow rate remains within optimal parameters.
By treating the FACS Sample Submission Form as a critical component of your experimental design rather than a simple administrative hurdle, you contribute to a more efficient research environment and significantly increase the quality and reproducibility of your flow cytometry data.
