Proteomics & Mass Spectrometry Core Facility and Reference File Download Link
https://eu2.contabostorage.com/00f3241116844f24b628f46d81abb929:st1/folder11/11970/13496_sample_submission_form.doc
2026-06-02 18:22:03 - Admin
<style> body { font-family: 'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; line-height: 1.6; color: #333; max-width: 900px; margin: 0 auto; padding: 20px; background-color: #ffffff; } h1 { color: #2c3e50; border-bottom: 2px solid #3498db; padding-bottom: 10px; } h2 { color: #2980b9; margin-top: 30px; } .intro { font-size: 1.1em; background-color: #f9f9f9; padding: 15px; border-left: 5px solid #3498db; } ul { padding-left: 20px; } </style> <h1>Proteomics & Mass Spectrometry Core Facility</h1> <div class="intro"> <p>The Proteomics & Mass Spectrometry Core Facility serves as a centralized research hub dedicated to the comprehensive study of the proteomethe entire set of proteins expressed by a genome, cell, tissue, or organism. By leveraging state-of-the-art mass spectrometry instrumentation, we provide the scientific community with the tools necessary to identify, quantify, and characterize proteins to advance biological and clinical research.</p> </div> <h2>Mission and Overview</h2> <p>Our facility is designed to bridge the gap between complex biological questions and analytical chemical solutions. Proteins are the functional workhorses of the cell, and their dysregulation is at the heart of most disease states, including cancer, neurodegeneration, and metabolic disorders. Through high-throughput analysis, our core provides investigators with critical insights into protein expression levels, post-translational modifications (PTMs), and protein-protein interactions.</p> <h2>Core Services</h2> <p>We offer a wide spectrum of analytical services tailored to the specific needs of modern molecular biology. Our primary workflows include:</p> <ul> <li><strong>Protein Identification:</strong> Utilizing Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) to identify proteins from complex mixtures, such as gel bands, cell lysates, or tissue extracts.</li> <li><strong>Quantitative Proteomics:</strong> Applying methodologies like TMT (Tandem Mass Tagging) labeling or Label-Free Quantitation (LFQ) to compare protein abundance across multiple biological conditions.</li> <li><strong>Post-Translational Modification (PTM) Analysis:</strong> Mapping dynamic modifications such as phosphorylation, acetylation, and ubiquitination, which are essential for understanding cell signaling and regulatory pathways.</li> <li><strong>Interactomics:</strong> Identifying binding partners through Affinity Purification Mass Spectrometry (AP-MS) to clarify the functional context of target proteins.</li> <li><strong>Intact Protein Analysis:</strong> Characterizing whole protein masses for quality control of recombinant proteins or therapeutic antibodies.</li> </ul> <h2>Instrumentation</h2> <p>Success in proteomics requires precision and sensitivity. Our facility maintains a fleet of high-end mass spectrometers, including:</p> <ul> <li>High-resolution, accurate-mass (HRAM) Orbitrap platforms for deep proteome coverage.</li> <li>Triple Quadrupole systems optimized for targeted proteomics and precise quantification of small molecules and peptides.</li> <li>Advanced nano-flow liquid chromatography systems for high-sensitivity sample delivery.</li> </ul> <h2>The Research Workflow</h2> <p>Engaging with the Core Facility typically follows a structured pipeline designed to maximize data quality:</p> <ol> <li><strong>Consultation:</strong> We begin with a project design meeting to define the biological question, determine the necessary sample preparation, and select the optimal analytical strategy.</li> <li><strong>Sample Preparation:</strong> Proper sample handling is vital. We provide guidance on protein extraction, digestion into peptides, and necessary cleanup steps to remove contaminants.</li> <li><strong>Data Acquisition:</strong> Samples are processed through our LC-MS/MS workflows under rigorous quality control standards.</li> <li><strong>Bioinformatics Analysis:</strong> Raw data is converted into biological knowledge using advanced software suites for protein identification, statistical analysis, and pathway enrichment mapping.</li> </ol> <h2>Why Choose Core Support?</h2> <p>Proteomics is a technically demanding field that requires expensive infrastructure and specialized bioinformatics expertise. By centralizing these resources, our core facility offers several advantages: economies of scale in reagent purchasing, consistent standardized operating procedures (SOPs), access to the latest technological breakthroughs, and dedicated staff to troubleshoot complex experimental setups. We are committed to fostering a collaborative environment that accelerates discovery and enables high-impact publications.</p> <h2>Collaboration and Training</h2> <p>Beyond providing raw data, we view ourselves as partners in your research. We regularly conduct training workshops on sample preparation techniques and data interpretation software. Our staff works closely with principal investigators to help refine study designs and assist with the proteomic sections of grant applications and manuscripts.</p>