Comprehensive solution for highquality hematoxylineosin stainingMMI H&E Staining Kit Plus
What Is the MMI H&E Staining Kit Plus?
The MMI H&E Staining Kit Plus is a readytouse, allinone kit designed for laboratories that need reliable, reproducible hematoxylin and eosin staining of paraffinembedded tissue sections. It combines optimized reagents, a standardized protocol, and qualitycontrolled slide handling instructions to deliver crisp, highcontrast histology images suitable for research, diagnostics, and teaching.
Key Components
- Hematoxylin Stock Solution (Gills 3step) provides consistent nuclear staining.
- Eosin Y Solution (0.5% in 95% ethanol) yields the characteristic pink cytoplasmic coloration.
- Rinsing Solutions including distilledwater rinse, acid alcohol, and bluing agent (Scotts tap water).
- Deparaffinization & Hydration Solutions xylene substitutes and graded ethanol series.
- Mounting Medium a clear, nonaqueous resin with antifade additives.
- Slidehandling accessories reusable staining dishes, coverslips, and a stepbystep protocol sheet.
Standard Protocol (25minute workflow)
- Deparaffinize slides in the provided xylene substitute (22min).
Note: Ensure complete removal of paraffin before proceeding. - Hydrate through graded ethanol (100%, 95%, 70%, each 30s) and rinse in distilled water.
- Stain nuclei with Hematoxylin for 5min. Rinse briefly in running tap water.
- Blueing in Scotts tap water for 30s to develop the distinct bluepurple nuclear hue.
- Eosin staining for 23min, depending on desired cytoplasmic intensity. Rinse in distilled water.
- Dehydrate through 95% ethanol (30s) and 100% ethanol (230s).
Optional: a quick dip in xylene substitute to clear the tissue. - Mount using the provided resin and place a coverslip. Allow to set for at least 5min before imaging.
Advantages Over Conventional H&E Kits
- Consistency reagent concentrations are preverified, reducing batchtobatch variability.
- Safety xylenefree deparaffinization reduces hazardous exposure.
- Speed streamlined workflow can be completed in under half an hour without compromising quality.
- Compatibility works with automated stainers as well as manual benchtop procedures.
- Longterm stability reagents retain efficacy for up to 12months when stored at 28C.
Applications
The MMI H&E Staining Kit Plus is suitable for a broad range of histological examinations, including:
- Pathology diagnostics clear differentiation of cellular architecture.
- Research immunohistochemistry (IHC) prestaining to assess tissue morphology.
- Teaching highcontrast slides ideal for histology courses and labs.
- Quality control reference slides for validating new staining protocols or equipment.
Troubleshooting Guide
| Issue | Possible Cause | Solution |
|---|---|---|
| Poor nuclear staining (light blue) | Insufficient hematoxylin exposure or aged reagent | Extend hematoxylin staining by 12min; replace the Hematoxylin if >12months old. |
| Excessive eosin background | Overstaining or incomplete dehydration | Shorten eosin time; ensure proper dehydration through 100% ethanol before mounting. |
| Uneven coloration across the slide | Unequal reagent distribution or uneven slide drying | Agitate staining dishes gently; confirm slides are fully flat on the staining rack. |
| Crystal formation on coverslip | Residues of water or ethanol trapped during mounting | Dry slides thoroughly after dehydration; use fresh mounting medium. |
Ordering Information
The kit is supplied in a single, tightly sealed package containing enough reagents for up to 250 standard slides. Each kit includes a QRcode that links to an online video tutorial and the latest safety data sheets.
- Catalog Number: MMIHEPLUS001
- Price (USD): $199.00 per kit (volume discounts available)
- Shipping: Free standard shipping within the United States; international shipping on request.
Orders can be placed through the MMI online store or by contacting the regional sales representative.
Frequently Asked Questions
- Q: Can the kit be used on frozen sections?
- A: While optimized for paraffinembedded tissue, the reagents are compatible with frozen sections. Adjust the deparaffinization step (skip) and shorten hydration times.
- Q: Is the mounting medium compatible with fluorescence microscopy?
- A: The standard resin is not fluorescentcompatible. For fluorescence applications, use a separate antifade mounting medium after H&E staining.
- Q: How long can stained slides be stored?
- A: Properly mounted slides retain their color and morphology for at least 2years when stored in a dry, dark environment.
- Q: Does the kit work with automated stainers?
- A: Yes. The kits reagent volumes and incubation times match the common programming settings of most automated H&E platforms.
Contact & Support
For technical assistance, please email support@mmi.com or call 18005550123 (MonFri, 8am6pm EST). Our team is ready to help you achieve the best possible histology results.
