General Guidelines for Sequencing Sample Submission
To ensure high-quality sequencing data and efficient turnaround times, please adhere to the following submission guidelines. Improperly prepared or labeled samples may lead to delays in processing or failure to achieve desired results.
1. Sample Preparation and Quality Control
Before submitting your samples, verify that they meet the minimum quality requirements for your specific sequencing platform. Common requirements include:
- Purity: Samples should be free of contaminants such as salts, ethanol, phenol, or protein. Using commercial purification kits is highly recommended.
- Quantification: Always measure concentration using a fluorometric method (e.g., Qubit) rather than spectrophotometry (e.g., NanoDrop), as fluorometric methods are more accurate for low-concentration samples.
- Integrity: If submitting RNA or high-molecular-weight DNA, ensure the integrity is checked via capillary electrophoresis (e.g., Agilent Bioanalyzer or TapeStation). An RNA Integrity Number (RIN) of 7.0 or higher is typically required for RNA-Seq.
2. Buffers and Solvents
Always resuspend your samples in the appropriate buffer as specified by the service provider. In most cases, 10 mM Tris-HCl (pH 8.0) or nuclease-free water is preferred. Avoid using buffers that contain EDTA at high concentrations, as it can interfere with downstream enzymatic reactions.
3. Labeling and Documentation
Proper documentation is vital for sample tracking and data management. Please ensure the following:
- Tube Labeling: Clearly label each tube with a unique identifier that matches the information provided in your submission form. Use permanent, chemical-resistant markers.
- Submission Forms: Complete all sections of the online or physical submission form. Include details regarding the source species, library type, and expected concentration.
- Inventory: Include a manifest or inventory sheet if submitting a large batch of samples.
4. Shipping and Storage
The shipping process is critical to maintaining sample stability:
- Cold Chain: Most DNA and RNA samples must be shipped on dry ice. Ensure the shipping container has a sufficient quantity of dry ice to last for the duration of the transit.
- Packaging: Use secondary containment for all tubes. Place tubes in a sturdy rack or box to prevent damage during movement.
- Scheduling: Ship samples early in the week (MondayWednesday) to prevent packages from being held in transit over the weekend.
5. Frequently Asked Questions
Can I submit multiple samples in a single tube?
No. All samples must be submitted in separate, clearly labeled microcentrifuge tubes or plates.
What happens if my samples do not meet quality standards?
If your samples fail initial quality control checks, a technician will contact you to discuss potential risks. You may choose to proceed at your own risk, provide replacement samples, or request a re-purification.
Do I need to send excess material?
While it is helpful to provide a slightly larger volume than required for the run, please do not send excessive amounts of hazardous materials. Only send what is needed for the requested service plus a small margin for contingency.
